Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

William Durand avatar William DurandTwishi Gulati avatar Twishi GulatiEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilAnton Nekrutenko avatar Anton NekrutenkoTorsten Seemann avatar Torsten SeemannHélène Chiapello avatar Hélène Chiapelloorcid logoDelphine Lariviere avatar Delphine LariviereNiall Beard avatar Niall BeardBazante Sanders avatar Bazante Sandersorcid logoMaria Doyle avatar Maria Doyleorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAlexandre Cormier avatar Alexandre CormierNathan Dunn avatar Nathan Dunnorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANEkaterina Polkh avatar Ekaterina PolkhGildas Le Corguillé avatar Gildas Le CorguilléSimon Bray avatar Simon Brayorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnna Syme avatar Anna Symeorcid logoErwan Corre avatar Erwan CorreJasper Ouwerkerk avatar Jasper OuwerkerkKenji Fujihara avatar Kenji Fujiharaorcid logoTeresa Müller avatar Teresa Müllerorcid logoHelena Rasche avatar Helena Rascheorcid logoBjörn Grüning avatar Björn Grüningorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoLaura Leroi avatar Laura Leroiorcid logoMiaomiao Zhou avatar Miaomiao ZhouValentin Loux avatar Valentin Louxorcid logoPaul Zierep avatar Paul Ziereporcid logoSimon Gladman avatar Simon Gladmanorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBérénice Batut avatar Bérénice Batutorcid logoMateo Boudet avatar Mateo BoudetGuillaume Gautreau avatar Guillaume Gautreauorcid logoNicola Soranzo avatar Nicola Soranzo

Funding

These individuals or organisations provided funding support for the development of this resource