Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Bazante Sanders avatar Bazante SandersKenji Fujihara avatar Kenji Fujiharaorcid logoSaim Momin avatar Saim Mominorcid logoMateo Boudet avatar Mateo Boudetorcid logoPaul Zierep avatar Paul Ziereporcid logoStéphanie Robin avatar Stéphanie Robinorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAlexandre Cormier avatar Alexandre CormierGuillaume Gautreau avatar Guillaume Gautreauorcid logoSaskia Hiltemann avatar Saskia HiltemannTorsten Seemann avatar Torsten Seemannorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoNiklas Mayle avatar Niklas MayleWilliam Durand avatar William DurandEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilSimon Bray avatar Simon BrayValentin Loux avatar Valentin Louxorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoFelicitas Kindel avatar Felicitas KindelHélène Chiapello avatar Hélène Chiapelloorcid logoTeresa Müller avatar Teresa Müllerorcid logoBjörn Grüning avatar Björn Grüningorcid logoMaria Doyle avatar Maria Doyleorcid logoLaura Leroi avatar Laura LeroiAnton Nekrutenko avatar Anton Nekrutenkoorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoDeepti Varshney avatar Deepti VarshneyNiall Beard avatar Niall BeardJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoMiaomiao Zhou avatar Miaomiao ZhouNathan Dunn avatar Nathan Dunnorcid logoErwan Corre avatar Erwan Correorcid logoVesselin Baev avatar Vesselin Baevorcid logoAnna Syme avatar Anna Symeorcid logoTristan Reynolds avatar Tristan ReynoldsTwishi Gulati avatar Twishi Gulatiorcid logoHelena Rasche avatar Helena RascheEkaterina Polkh avatar Ekaterina Polkhorcid logoJonathan Kreplak avatar Jonathan KreplakGildas Le Corguillé avatar Gildas Le Corguilléorcid logoSimon Gladman avatar Simon Gladmanorcid logoBérénice Batut avatar Bérénice Batut

Funding

These individuals or organisations provided funding support for the development of this resource