Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBérénice Batut avatar Bérénice Batutorcid logoJonathan Kreplak avatar Jonathan KreplakNiall Beard avatar Niall Beardorcid logoDelphine Lariviere avatar Delphine LariviereBazante Sanders avatar Bazante Sandersorcid logoErwan Corre avatar Erwan Correorcid logoMaria Doyle avatar Maria Doyleorcid logoMateo Boudet avatar Mateo Boudetorcid logoHelena Rasche avatar Helena Rascheorcid logoPaul Zierep avatar Paul Ziereporcid logoNiklas Mayle avatar Niklas MayleEkaterina Polkh avatar Ekaterina Polkhorcid logoAnna Syme avatar Anna Symeorcid logoSaskia Hiltemann avatar Saskia HiltemannHélène Chiapello avatar Hélène ChiapelloWilliam Durand avatar William DurandAnton Nekrutenko avatar Anton NekrutenkoTwishi Gulati avatar Twishi Gulatiorcid logoCristóbal Gallardo avatar Cristóbal GallardoValentin Loux avatar Valentin LouxNathan Dunn avatar Nathan Dunnorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoTeresa Müller avatar Teresa MüllerTorsten Seemann avatar Torsten SeemannSimon Bray avatar Simon Brayorcid logoBjörn Grüning avatar Björn GrüningGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSimon Gladman avatar Simon GladmanEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoNicola Soranzo avatar Nicola SoranzoGuillaume Gautreau avatar Guillaume Gautreauorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoLaura Leroi avatar Laura Leroiorcid logoSaim Momin avatar Saim Mominorcid logoFelicitas Kindel avatar Felicitas KindelKenji Fujihara avatar Kenji Fujiharaorcid logoAlexandre Cormier avatar Alexandre CormierJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoStéphanie Robin avatar Stéphanie Robin

Funding

These individuals or organisations provided funding support for the development of this resource