Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoSimon Gladman avatar Simon Gladmanorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnika Erxleben avatar Anika ErxlebenTorsten Seemann avatar Torsten Seemannorcid logoMateo Boudet avatar Mateo Boudetorcid logoAnna Syme avatar Anna SymeHélène Chiapello avatar Hélène Chiapelloorcid logoDeepti Varshney avatar Deepti VarshneyAnton Nekrutenko avatar Anton Nekrutenkoorcid logoLaura Leroi avatar Laura LeroiKenji Fujihara avatar Kenji FujiharaJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNathan Dunn avatar Nathan DunnTwishi Gulati avatar Twishi GulatiBazante Sanders avatar Bazante SandersGildas Le Corguillé avatar Gildas Le Corguilléorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoBjörn Grüning avatar Björn GrüningSimon Bray avatar Simon Brayorcid logoStéphanie Robin avatar Stéphanie RobinWilliam Durand avatar William Durandorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoDelphine Lariviere avatar Delphine LariviereEkaterina Polkh avatar Ekaterina Polkhorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoBérénice Batut avatar Bérénice BatutNiall Beard avatar Niall Beardorcid logoCristóbal Gallardo avatar Cristóbal GallardoValentin Loux avatar Valentin Louxorcid logoFelicitas Kindel avatar Felicitas KindelGuillaume Gautreau avatar Guillaume Gautreauorcid logoJonathan Kreplak avatar Jonathan KreplakEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoErwan Corre avatar Erwan Correorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoMaria Doyle avatar Maria Doyleorcid logoPaul Zierep avatar Paul Zierep

Funding

These individuals or organisations provided funding support for the development of this resource