Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoTeresa Müller avatar Teresa Müllerorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaim Momin avatar Saim Mominorcid logoPaul Zierep avatar Paul Ziereporcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoNiklas Mayle avatar Niklas Mayleorcid logoErwan Corre avatar Erwan Correorcid logoAlexandre Cormier avatar Alexandre CormierValentin Loux avatar Valentin Louxorcid logoDeepti Varshney avatar Deepti VarshneyJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBérénice Batut avatar Bérénice Batutorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoTristan Reynolds avatar Tristan ReynoldsGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMaria Doyle avatar Maria Doyleorcid logoAnna Syme avatar Anna SymeEkaterina Polkh avatar Ekaterina Polkhorcid logoCristóbal Gallardo avatar Cristóbal GallardoSimon Bray avatar Simon BrayGuillaume Gautreau avatar Guillaume Gautreauorcid logoLaura Leroi avatar Laura Leroiorcid logoMateo Boudet avatar Mateo Boudetorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William DurandEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoJonathan Kreplak avatar Jonathan KreplakTwishi Gulati avatar Twishi Gulatiorcid logoMiaomiao Zhou avatar Miaomiao ZhouNiall Beard avatar Niall Beardorcid logoBjörn Grüning avatar Björn Grüningorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoSimon Gladman avatar Simon GladmanNathan Dunn avatar Nathan Dunnorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoHelena Rasche avatar Helena RascheBazante Sanders avatar Bazante SandersTorsten Seemann avatar Torsten Seemannorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoStéphanie Robin avatar Stéphanie RobinKenji Fujihara avatar Kenji FujiharaHélène Chiapello avatar Hélène ChiapelloAnton Nekrutenko avatar Anton Nekrutenko

Funding

These individuals or organisations provided funding support for the development of this resource