Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoBérénice Batut avatar Bérénice Batutorcid logoPaul Zierep avatar Paul Ziereporcid logoAnika Erxleben avatar Anika Erxlebenorcid logoLaura Leroi avatar Laura LeroiNathan Dunn avatar Nathan DunnEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilTwishi Gulati avatar Twishi GulatiDeepti Varshney avatar Deepti Varshneyorcid logoMaria Doyle avatar Maria DoyleGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna Symeorcid logoNicola Soranzo avatar Nicola SoranzoEkaterina Polkh avatar Ekaterina PolkhAnton Nekrutenko avatar Anton NekrutenkoBazante Sanders avatar Bazante Sandersorcid logoCristóbal Gallardo avatar Cristóbal GallardoTorsten Seemann avatar Torsten SeemannWilliam Durand avatar William Durandorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAlexandre Cormier avatar Alexandre CormierJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoAnthony Bretaudeau avatar Anthony BretaudeauKenji Fujihara avatar Kenji FujiharaNiall Beard avatar Niall Beardorcid logoSimon Gladman avatar Simon GladmanSimon Bray avatar Simon Brayorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoBjörn Grüning avatar Björn Grüningorcid logoErwan Corre avatar Erwan CorreTeresa Müller avatar Teresa Müllerorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoMateo Boudet avatar Mateo Boudetorcid logoHelena Rasche avatar Helena Rascheorcid logoDelphine Lariviere avatar Delphine Lariviere

Funding

These individuals or organisations provided funding support for the development of this resource