Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

For any question or discussions related to this topic, or to connect with others in the community, please visit the community chat:

comment   Community Matrix Chat

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

Follow topic updates rss-feed with our RSS Feed

Community Resources

Community Home Maintainer Home

Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Are you on the editorial board or want to help out with this topic? View the topic maintainer page for ways to improve this topic!

Contributors

This material was contributed to by:

orcid logoMateo Boudet avatar Mateo BoudetEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilTorsten Seemann avatar Torsten Seemannorcid logoRomane LIBOUBAN avatar Romane LIBOUBANNiall Beard avatar Niall Beardorcid logoPaul Zierep avatar Paul Ziereporcid logoLaura Leroi avatar Laura Leroiorcid logoAnthony Bretaudeau avatar Anthony BretaudeauKenji Fujihara avatar Kenji Fujiharaorcid logoBjörn Grüning avatar Björn Grüningorcid logoErwan Corre avatar Erwan CorreSimon Bray avatar Simon BrayGildas Le Corguillé avatar Gildas Le CorguilléTwishi Gulati avatar Twishi Gulatiorcid logoStéphanie Robin avatar Stéphanie RobinValentin Loux avatar Valentin Louxorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoSimon Gladman avatar Simon GladmanBazante Sanders avatar Bazante SandersGuillaume Gautreau avatar Guillaume Gautreauorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoTeresa Müller avatar Teresa Müllerorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoHelena Rasche avatar Helena Rascheorcid logoSaim Momin avatar Saim Mominorcid logoMaria Doyle avatar Maria Doyleorcid logoNicola Soranzo avatar Nicola SoranzoAnton Nekrutenko avatar Anton Nekrutenkoorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoAlexandre Cormier avatar Alexandre CormierHélène Chiapello avatar Hélène ChiapelloNathan Dunn avatar Nathan Dunnorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnna Syme avatar Anna Symeorcid logoBérénice Batut avatar Bérénice BatutEkaterina Polkh avatar Ekaterina PolkhJasper Ouwerkerk avatar Jasper OuwerkerkWilliam Durand avatar William Durandorcid logoNiklas Mayle avatar Niklas Mayleorcid logoFelicitas Kindel avatar Felicitas Kindel

Funding

These individuals or organisations provided funding support for the development of this resource