Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

For any question or discussions related to this topic, or to connect with others in the community, please visit the community chat:

comment   Community Matrix Chat

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

Follow topic updates rss-feed with our RSS Feed

Community Resources

Community Home Maintainer Home

Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Are you on the editorial board or want to help out with this topic? View the topic maintainer page for ways to improve this topic!

Contributors

This material was contributed to by:

Torsten Seemann avatar Torsten Seemannorcid logoMiaomiao Zhou avatar Miaomiao ZhouNiall Beard avatar Niall BeardSimon Bray avatar Simon Brayorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANWilliam Durand avatar William DurandGildas Le Corguillé avatar Gildas Le CorguilléHélène Chiapello avatar Hélène ChiapelloEkaterina Polkh avatar Ekaterina PolkhKenji Fujihara avatar Kenji Fujiharaorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnna Syme avatar Anna Symeorcid logoPaul Zierep avatar Paul Ziereporcid logoLaura Leroi avatar Laura LeroiNathan Dunn avatar Nathan DunnJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBjörn Grüning avatar Björn Grüningorcid logoMaria Doyle avatar Maria Doyleorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnika Erxleben avatar Anika ErxlebenBazante Sanders avatar Bazante SandersGuillaume Gautreau avatar Guillaume GautreauTwishi Gulati avatar Twishi Gulatiorcid logoJonathan Kreplak avatar Jonathan KreplakValentin Loux avatar Valentin Louxorcid logoBérénice Batut avatar Bérénice Batutorcid logoHelena Rasche avatar Helena Rascheorcid logoNicola Soranzo avatar Nicola SoranzoAnton Nekrutenko avatar Anton Nekrutenkoorcid logoTeresa Müller avatar Teresa Müllerorcid logoErwan Corre avatar Erwan Correorcid logoCristóbal Gallardo avatar Cristóbal GallardoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMateo Boudet avatar Mateo Boudet

Funding

These individuals or organisations provided funding support for the development of this resource