Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoBjörn Grüning avatar Björn Grüningorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoHelena Rasche avatar Helena Rascheorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoMiaomiao Zhou avatar Miaomiao ZhouTwishi Gulati avatar Twishi GulatiKenji Fujihara avatar Kenji Fujiharaorcid logoSaim Momin avatar Saim MominJasper Ouwerkerk avatar Jasper OuwerkerkHélène Chiapello avatar Hélène Chiapelloorcid logoTeresa Müller avatar Teresa MüllerNathan Dunn avatar Nathan Dunnorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoPaul Zierep avatar Paul ZierepSimon Bray avatar Simon Brayorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMateo Boudet avatar Mateo Boudetorcid logoErwan Corre avatar Erwan Correorcid logoAnna Syme avatar Anna SymeGuillaume Gautreau avatar Guillaume Gautreauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAlexandre Cormier avatar Alexandre CormierTorsten Seemann avatar Torsten SeemannWilliam Durand avatar William Durandorcid logoSimon Gladman avatar Simon Gladmanorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoNiklas Mayle avatar Niklas MayleEkaterina Polkh avatar Ekaterina PolkhBazante Sanders avatar Bazante Sandersorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBérénice Batut avatar Bérénice BatutNiall Beard avatar Niall BeardEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDeepti Varshney avatar Deepti VarshneyValentin Loux avatar Valentin Louxorcid logoMaria Doyle avatar Maria DoyleGildas Le Corguillé avatar Gildas Le Corguilléorcid logoLaura Leroi avatar Laura LeroiAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Funding

These individuals or organisations provided funding support for the development of this resource