Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Jasper Ouwerkerk avatar Jasper OuwerkerkSimon Bray avatar Simon Brayorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnna Syme avatar Anna SymeTwishi Gulati avatar Twishi GulatiKenji Fujihara avatar Kenji FujiharaWilliam Durand avatar William DurandEkaterina Polkh avatar Ekaterina Polkhorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaskia Hiltemann avatar Saskia HiltemannValentin Loux avatar Valentin Louxorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAnika Erxleben avatar Anika ErxlebenAnton Nekrutenko avatar Anton NekrutenkoNiall Beard avatar Niall BeardGuillaume Gautreau avatar Guillaume Gautreauorcid logoHelena Rasche avatar Helena RascheGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoBjörn Grüning avatar Björn GrüningHélène Chiapello avatar Hélène ChiapelloEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAlexandre Cormier avatar Alexandre CormierTorsten Seemann avatar Torsten Seemannorcid logoBérénice Batut avatar Bérénice Batutorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoMaria Doyle avatar Maria Doyleorcid logoLaura Leroi avatar Laura Leroiorcid logoErwan Corre avatar Erwan Correorcid logoStéphanie Robin avatar Stéphanie RobinBazante Sanders avatar Bazante Sandersorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoPaul Zierep avatar Paul Ziereporcid logoMateo Boudet avatar Mateo BoudetNathan Dunn avatar Nathan Dunnorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoJonathan Kreplak avatar Jonathan Kreplak

Funding

These individuals or organisations provided funding support for the development of this resource