Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoErwan Corre avatar Erwan Correorcid logoAnthony Bretaudeau avatar Anthony BretaudeauTwishi Gulati avatar Twishi Gulatiorcid logoMateo Boudet avatar Mateo Boudetorcid logoAnna Syme avatar Anna SymeGildas Le Corguillé avatar Gildas Le CorguilléEkaterina Polkh avatar Ekaterina Polkhorcid logoFelicitas Kindel avatar Felicitas KindelBazante Sanders avatar Bazante SandersValentin Loux avatar Valentin Louxorcid logoMaria Doyle avatar Maria DoyleSimon Bray avatar Simon BrayNiall Beard avatar Niall Beardorcid logoBérénice Batut avatar Bérénice BatutJasper Ouwerkerk avatar Jasper OuwerkerkTorsten Seemann avatar Torsten Seemannorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoPaul Zierep avatar Paul ZierepGuillaume Gautreau avatar Guillaume Gautreauorcid logoSimon Gladman avatar Simon Gladmanorcid logoTeresa Müller avatar Teresa Müllerorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBjörn Grüning avatar Björn GrüningNathan Dunn avatar Nathan DunnEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoLaura Leroi avatar Laura Leroiorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoHelena Rasche avatar Helena RascheWilliam Durand avatar William Durandorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoSaskia Hiltemann avatar Saskia HiltemannHélène Chiapello avatar Hélène ChiapelloKenji Fujihara avatar Kenji FujiharaAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoJonathan Kreplak avatar Jonathan Kreplak

Funding

These individuals or organisations provided funding support for the development of this resource