Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMateo Boudet avatar Mateo Boudetorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoMaria Doyle avatar Maria Doyleorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoErwan Corre avatar Erwan CorreWilliam Durand avatar William Durandorcid logoTeresa Müller avatar Teresa Müllerorcid logoLaura Leroi avatar Laura Leroiorcid logoHelena Rasche avatar Helena Rascheorcid logoAnika Erxleben avatar Anika ErxlebenEkaterina Polkh avatar Ekaterina Polkhorcid logoFelicitas Kindel avatar Felicitas KindelNathan Dunn avatar Nathan DunnNiall Beard avatar Niall Beardorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBjörn Grüning avatar Björn Grüningorcid logoRomane LIBOUBAN avatar Romane LIBOUBANJasper Ouwerkerk avatar Jasper OuwerkerkGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna Symeorcid logoSimon Gladman avatar Simon GladmanTorsten Seemann avatar Torsten Seemannorcid logoDelphine Lariviere avatar Delphine LariviereEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoAlexandre Cormier avatar Alexandre CormierValentin Loux avatar Valentin Louxorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBérénice Batut avatar Bérénice BatutSimon Bray avatar Simon Brayorcid logoCristóbal Gallardo avatar Cristóbal GallardoAnton Nekrutenko avatar Anton NekrutenkoGuillaume Gautreau avatar Guillaume Gautreauorcid logoSaskia Hiltemann avatar Saskia HiltemannBazante Sanders avatar Bazante SandersKenji Fujihara avatar Kenji Fujiharaorcid logoPaul Zierep avatar Paul ZierepTwishi Gulati avatar Twishi GulatiHélène Chiapello avatar Hélène Chiapelloorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Funding

These individuals or organisations provided funding support for the development of this resource