Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Hélène Chiapello avatar Hélène Chiapelloorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoDeepti Varshney avatar Deepti VarshneyTwishi Gulati avatar Twishi GulatiValentin Loux avatar Valentin Louxorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoJonathan Kreplak avatar Jonathan KreplakNathan Dunn avatar Nathan Dunnorcid logoSimon Gladman avatar Simon Gladmanorcid logoStéphanie Robin avatar Stéphanie RobinEkaterina Polkh avatar Ekaterina Polkhorcid logoBjörn Grüning avatar Björn Grüningorcid logoHelena Rasche avatar Helena RascheBazante Sanders avatar Bazante SandersTorsten Seemann avatar Torsten Seemannorcid logoSaim Momin avatar Saim Mominorcid logoBérénice Batut avatar Bérénice BatutGuillaume Gautreau avatar Guillaume Gautreauorcid logoDelphine Lariviere avatar Delphine LariviereWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao ZhouNiall Beard avatar Niall Beardorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoLaura Leroi avatar Laura LeroiEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoTeresa Müller avatar Teresa Müllerorcid logoPaul Zierep avatar Paul Ziereporcid logoAnika Erxleben avatar Anika Erxlebenorcid logoFelicitas Kindel avatar Felicitas KindelJasper Ouwerkerk avatar Jasper OuwerkerkSimon Bray avatar Simon Brayorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANAnton Nekrutenko avatar Anton Nekrutenkoorcid logoNiklas Mayle avatar Niklas Mayleorcid logoErwan Corre avatar Erwan CorreGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMaria Doyle avatar Maria DoyleKenji Fujihara avatar Kenji Fujiharaorcid logoMateo Boudet avatar Mateo Boudetorcid logoAnna Syme avatar Anna Symeorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Funding

These individuals or organisations provided funding support for the development of this resource