Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAlexandre Cormier avatar Alexandre CormierWilliam Durand avatar William Durandorcid logoDeepti Varshney avatar Deepti VarshneyEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilValentin Loux avatar Valentin Louxorcid logoSaim Momin avatar Saim MominGildas Le Corguillé avatar Gildas Le Corguilléorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoJonathan Kreplak avatar Jonathan KreplakTorsten Seemann avatar Torsten SeemannNathan Dunn avatar Nathan Dunnorcid logoStéphanie Robin avatar Stéphanie RobinTwishi Gulati avatar Twishi Gulatiorcid logoErwan Corre avatar Erwan Correorcid logoPaul Zierep avatar Paul ZierepKenji Fujihara avatar Kenji Fujiharaorcid logoMateo Boudet avatar Mateo BoudetSimon Bray avatar Simon Brayorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoHelena Rasche avatar Helena Rascheorcid logoAnna Syme avatar Anna SymeGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa MüllerBazante Sanders avatar Bazante Sandersorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoBjörn Grüning avatar Björn Grüningorcid logoSaskia Hiltemann avatar Saskia HiltemannNiall Beard avatar Niall Beardorcid logoSimon Gladman avatar Simon Gladmanorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoNicola Soranzo avatar Nicola SoranzoAnton Nekrutenko avatar Anton Nekrutenkoorcid logoBérénice Batut avatar Bérénice Batutorcid logoMaria Doyle avatar Maria DoyleHélène Chiapello avatar Hélène ChiapelloEkaterina Polkh avatar Ekaterina Polkhorcid logoLaura Leroi avatar Laura Leroi

Funding

These individuals or organisations provided funding support for the development of this resource