Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

William Durand avatar William Durandorcid logoTeresa Müller avatar Teresa MüllerNiall Beard avatar Niall Beardorcid logoBjörn Grüning avatar Björn GrüningTwishi Gulati avatar Twishi Gulatiorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnika Erxleben avatar Anika ErxlebenEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilAnton Nekrutenko avatar Anton Nekrutenkoorcid logoHelena Rasche avatar Helena Rascheorcid logoErwan Corre avatar Erwan Correorcid logoJonathan Kreplak avatar Jonathan KreplakGuillaume Gautreau avatar Guillaume Gautreauorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoMaria Doyle avatar Maria Doyleorcid logoLaura Leroi avatar Laura Leroiorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoTristan Reynolds avatar Tristan ReynoldsKenji Fujihara avatar Kenji Fujiharaorcid logoSaim Momin avatar Saim MominBazante Sanders avatar Bazante SandersTorsten Seemann avatar Torsten Seemannorcid logoBérénice Batut avatar Bérénice Batutorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANHélène Chiapello avatar Hélène Chiapelloorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoDeepti Varshney avatar Deepti VarshneySimon Bray avatar Simon BrayJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoSaskia Hiltemann avatar Saskia HiltemannValentin Loux avatar Valentin Louxorcid logoSimon Gladman avatar Simon Gladmanorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoPaul Zierep avatar Paul ZierepNathan Dunn avatar Nathan DunnGildas Le Corguillé avatar Gildas Le Corguilléorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMateo Boudet avatar Mateo Boudetorcid logoAnna Syme avatar Anna Symeorcid logoAnthony Bretaudeau avatar Anthony BretaudeauEkaterina Polkh avatar Ekaterina Polkh

Funding

These individuals or organisations provided funding support for the development of this resource