Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoPaul Zierep avatar Paul Ziereporcid logoMaria Doyle avatar Maria Doyleorcid logoBjörn Grüning avatar Björn Grüningorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoTeresa Müller avatar Teresa Müllerorcid logoLaura Leroi avatar Laura Leroiorcid logoStéphanie Robin avatar Stéphanie RobinEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoRomane LIBOUBAN avatar Romane LIBOUBANSimon Bray avatar Simon Brayorcid logoAnika Erxleben avatar Anika ErxlebenWilliam Durand avatar William Durandorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoErwan Corre avatar Erwan CorreAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMateo Boudet avatar Mateo BoudetBazante Sanders avatar Bazante SandersValentin Loux avatar Valentin LouxKenji Fujihara avatar Kenji Fujiharaorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoNicola Soranzo avatar Nicola SoranzoTwishi Gulati avatar Twishi GulatiGildas Le Corguillé avatar Gildas Le CorguilléNiall Beard avatar Niall Beardorcid logoAnna Syme avatar Anna Symeorcid logoBérénice Batut avatar Bérénice Batutorcid logoMiaomiao Zhou avatar Miaomiao ZhouGuillaume Gautreau avatar Guillaume GautreauTorsten Seemann avatar Torsten SeemannHélène Chiapello avatar Hélène Chiapelloorcid logoAlexandre Cormier avatar Alexandre CormierEkaterina Polkh avatar Ekaterina Polkhorcid logoSimon Gladman avatar Simon GladmanJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoDeepti Varshney avatar Deepti VarshneyNathan Dunn avatar Nathan Dunnorcid logoHelena Rasche avatar Helena Rasche

Funding

These individuals or organisations provided funding support for the development of this resource