Champs blocs indicators
Author(s) | Marie Josse Yvan Le Bras |
Reviewers |
OverviewQuestions:Objectives:
Which biological questions are addressed by the tutorial?
Which bioinformatics techniques are important to know for this type of data?
Requirements:
Calculate IVR (Visual Rollover Indicator)
Compute dissimilarity indice
Vizualize some diversity indices
Time estimation: 1 hourSupporting Materials:Published: Jan 4, 2023Last modification: Sep 19, 2024License: Tutorial Content is licensed under Creative Commons Attribution 4.0 International License. The GTN Framework is licensed under MITpurl PURL: https://gxy.io/GTN:T00127version Revision: 10
This tutorial will allow you to better apprehend the workflow for indicators on boulder fields. This will help you understand the effects of global and local changes on marine habitats, and the effectiveness of the management measures adopted, requires standardized monitoring and robust and sensitive indicators reflecting the state of the habitats.
The “mediolittoral boulder fields” habitat harbors a high diversity of micro-habitats and species due to its high structural heterogeneity and its intermediate position on the foreshore, which makes it one of the most diversified mediolittoral habitats and of high ecological interest along the French Channel-Atlantic coast. It is also a very attractive habitat for recreational fishing, which, through the reworking of the blocks, can impact the communities. Thus, the “mediolittoral boulder fields” habitat has been the subject of several national and local initiatives (including LIFE+ “Experimentation for a sustainable and concerted management of recreational foot fishing in France” 2013-2017) to better assess its status and relate it to the pressure of foot fishing with a view to adapting local management, particularly through the network of Marine Protected Areas (Natura 2000, PNM, PNR etc.).
In particular, these projects have made it possible to develop a network of involved field players and managers and tools for evaluating the ecological status and pressure of fishing on foot:
- the Visual Boulder Turning Indicator (VTI), which is similar to a “landscape” indicator to assess fishing pressure based on architectural criteria;
- the Boulder Field Ecological Quality Index (BFEQ) - the subject of this report - based on biotic and abiotic variables that respond to the disturbance “boulder turning”.
Here we will go over the different steps in order to get those 2 indicators and more.
AgendaIn this tutorial, we will cover:
You can leave the tutorial and go bask on the main screen by clicking outside of the tutorial screen. You can come back to where you left off the tutorial anytime by clicking level.
Hands-on: Log in to Galaxy
- Open your favorite browser (Chrome, Safari or Firefox as your browser, not Internet Explorer!)
- Browse to your Galaxy instance
- Log in or register
This is an image of Galaxy Ecology, located at usegalaxy.eu
The Galaxy homepage is divided into three panels:
- Tools on the left
- Viewing panel in the middle
- History of analysis and files on the right
The first time you use Galaxy, there will be no files in your history panel.
Now let’s focus on our workflow on boulder field ecological state
Get data
Hands-on: Choose Your Own TutorialThis is a "Choose Your Own Tutorial" section, where you can select between multiple paths. Click one of the buttons below to select how you want to follow the tutorial
Are your ESTAMP data ready ?
- Download your data on ESTAMP estamp.afbiodiversite.fr website, clicking on “Accédez aux données” at the bottom of the page. You will get a zip folder.
-
Unzip your folder. In the folder three files .csv will interest us :
- champbloc_ivr.csv
- champbloc_qecb.csv
- ficheterrain.csv
Hands-on: Data upload
- Import the data into Galaxy
- Open the Galaxy Upload Manager galaxy-upload
- Select Choose local files
Browse in your computer and get your ESTAMP files (select all three of them : champbloc_ivr.csv, champbloc_qecb.csv and ficheterrain.csv)
- Press Start
- Press Close
You need to wait for the data to become green on the right panel
Create a new history for this tutorial and give it a name (example: “Champs blocs indicators”) for you to find it again later if needed. <p>To create a new history simply click the new-history icon at the top of the history panel:</p> <p></p>
Computing the Visual Boulder turning Indicator for each site
Based on the proportion of “turned” and “not turned” blocs this indicator vary from 0 to 5 and can be quickly used.
Hands-on: Use a tool
- click on for example the IVR ( Galaxy version 0.0.0) tool or type ivr in the tools panel search box (top left) The tool form will be displayed in the central Galaxy panel.
IVR
Go on the tool IVR
Hands-on: Calculating IVR
- IVR ( Galaxy version 0.0.0) with the following parameters:
- param-file “Input champbloc_ivr.csv”:
input
(champbloc_ivr.csv)- param-file “Input ficheterrain.csv”:
input
(ficheterrain.csv)Press Run Tool (it can take some minutes to process it’s normal if you have to wait a bit especially if your internet connection is weak)
Three outputs will appear in your history panel on the right.
- View graphs
- Once it’s green, click on IVR plot
- Then, click on the galaxy-eye (eye) icon of your output in the history panel.
The information is displayed in the central panel
- In the top of the History panel (on the right) go on the search box
- Click on the double arrows to display more filter options
- Type the name of your site (for instance “Bilfot”) on the “Filter by name:” criteria then click on “No” for the “Visible:” criteria, as plots are created in a data collection and not directly visible on the history
For your reports you need to download the ones you want. You won’t be able to vizualise them directly on Galaxy center panel !
- Download results
- Click on the output you are interested in for instance Reports
- Click on galaxy-save (download)
Computing dissimilarity coefficient for each site
Dissimilarity
Cleaning out your data in order and then compute de dissimilarity coefficient. Get the Dissimilarity ( Galaxy version 0.0.0) tool
Hands-on: Calculate dissimilarity index
- Dissimilarity ( Galaxy version 0.0.0) with the following parameters:
- param-file “Input champbloc_qecb.csv”:
input
(champbloc_qecb.csv)param-file “Input ficheterrain.csv”:
input
(ficheterrain.csv)- param-select “Do you have data after the year 2021 ?”:
No
- param-text “Until when do you have data (write only the YEAR) ?”:
2021
Press Run Tool (it can take some minutes to process it’s normal if you have to wait a bit especially if your internet connection is weak)
- View graphs
- Once it’s green, click on type of plot you want to see
- Then, click on the galaxy-eye (eye) icon of your output in the history panel.
The information is displayed in the central panel
- Download your results
- Click on the output you are interested in
- Click on galaxy-save (download)
Diversity indicators
Taking as input cleaned data from the Dissimilarity step tocompute diversity indicators. Get the Diversity indicators ( Galaxy version 0.0.0) tool
Hands-on: Calculate diversity indicators
- Diversity indicators ( Galaxy version 0.0.0) with the following parameters:
- param-file “Input clean data”:
input
(Clean Rdata)Press Execute
- View graphs
- Once it’s green, click on type of plot you want to see
- Then, click on the galaxy-eye (eye) icon of your output in the history panel.
The information is displayed in the central panel
- Download your results
- Click on the output you are interested in
- Click on galaxy-save (download)
Conclusion
Here you have finished your study on your boulder fields biodiversity state.